Journal: bioRxiv

Article Title: Dynamic and Heterogeneous Distribution of Trichomonasvirus Species in Trichomonas vaginalis

doi: 10.1101/2025.03.31.646274

Figure Lengend Snippet: The presence of TVV in the T. vaginalis isolates (ATCC 30236, ATCC 50143, ATCC 50148, ATCC PRA-98) was determined by mapping RNA-seq reads to Trichomonasvirus reference sequences obtained from the NCBI Virus Database using QIAGEN CLC Genomics Workbench (v20.0.3). (A) Summary of consensus coverage, average coverage, and total read counts. (B) Schematic representation of the consensus contig alignment for each Trichomonasvirus species. (C) Detection of Trichomonasvirus isolates using RT-PCR with virus species-specific primers, with PCR products separated on a 1% agarose gel.

Article Snippet: The T. vaginalis isolates (ATCC 30236, ATCC 50143, ATCC 50148, ATCC PRA-98) used in this study were obtained from the American Type Culture Collection (ATCC) in Manassas, Virginia, USA.

Techniques: RNA Sequencing, Virus, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis

Journal: bioRxiv

Article Title: Dynamic and Heterogeneous Distribution of Trichomonasvirus Species in Trichomonas vaginalis

doi: 10.1101/2025.03.31.646274

Figure Lengend Snippet: (A) Upset plots illustrate the distribution of viral populations among the T. vaginalis isolates (ATCC 30236, ATCC PRA-98, ATCC 50148, and ATCC 50143) using scRNA-seq. The plots show the presence of Trichomonasvirus species and their co-occurrence patterns across isolates. The bar graphs indicate the total number of reads mapped to each viral species in individual isolates. (B) Detection of viral dsRNA using the dsRNA-specific antibody 9D5 in different T. vaginalis isolates. Nuclei were stained with DAPI (blue), while viral dsRNA was detected using the 9D5 antibody conjugated with Alexa Fluor 594 (red). Images indicate the heterogenicity of viral dsRNA within the parasite. Scale bars = 10 µm.

Article Snippet: The T. vaginalis isolates (ATCC 30236, ATCC 50143, ATCC 50148, ATCC PRA-98) used in this study were obtained from the American Type Culture Collection (ATCC) in Manassas, Virginia, USA.

Techniques: Staining

Journal: bioRxiv

Article Title: Dynamic and Heterogeneous Distribution of Trichomonasvirus Species in Trichomonas vaginalis

doi: 10.1101/2025.03.31.646274

Figure Lengend Snippet: High-resolution scRNA-seq data from the T. vaginalis isolate ATCC 30236 was visualized using Loupe Browser 8.0.0, with uniform manifold approximation and projection (UMAP) dimensionality reduction. Each point represents an individual cell, with color intensity indicating the expression levels of viral-associated proteins, quantified as unique molecular identifiers (UMI). The scale bar reflects the transcript abundance distribution, highlighting the heterogeneity in viral gene expression across the population.

Article Snippet: The T. vaginalis isolates (ATCC 30236, ATCC 50143, ATCC 50148, ATCC PRA-98) used in this study were obtained from the American Type Culture Collection (ATCC) in Manassas, Virginia, USA.

Techniques: Expressing, Gene Expression

Journal: Biomedicines

Article Title: Dissecting the Transcriptomes of Multiple Metronidazole-Resistant and Sensitive Trichomonas vaginalis Strains Identified Distinct Genes and Pathways Associated with Drug Resistance and Cell Death

doi: 10.3390/biomedicines9121817

Figure Lengend Snippet: Shared DEGs in all MTZ-R transcriptomes compared with those of MTZ-S in the presence of MTZ.

Article Snippet: The T. vaginalis MTZ-S strains (ATCC 30001, ATCC 30236, and ATCC50148) and MTZ-R strains (ATCC 50142, ATCC 50143, and ATCC 30238) were maintained in YIS medium, pH 5.8, containing 10% heat-inactivated horse serum and 1% glucose at 37 °C.

Techniques: Ubiquitin Proteomics

Journal: Journal of Clinical Laboratory Analysis

Article Title: The Performance of the Abbott i2000 for Measuring Serum Markers of Infectious Diseases

doi: 10.1002/jcla.22015

Figure Lengend Snippet: The Results of 20 Times Measuring for LOD and LOQ Sample

Article Snippet: Twenty samples from China's National Center for Clinical Laboratory Proficiency Testing that covered the detection range of the Abbott i2000 for HBsAg and HBsAb were selected and assessed using five Abbott i2000 machines (serial numbers: 52691, 52693, 52694, 50143, and 54290).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of Clinical Laboratory Analysis

Article Title: The Performance of the Abbott i2000 for Measuring Serum Markers of Infectious Diseases

doi: 10.1002/jcla.22015

Figure Lengend Snippet: Evaluation of Consistency for HBsAg and HBsAb

Article Snippet: Twenty samples from China's National Center for Clinical Laboratory Proficiency Testing that covered the detection range of the Abbott i2000 for HBsAg and HBsAb were selected and assessed using five Abbott i2000 machines (serial numbers: 52691, 52693, 52694, 50143, and 54290).

Techniques: